Rapid serological identification of Vibrio vulnificus by anti-H coagglutination
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چکیده
منابع مشابه
RAPID IDENTIFICATION OF Vibrio cholera-01 BY COAGGLUTINATION TEST USING MONO-SPECIFIC ANTIBODY
Mono-specific antisera against Vibrio cholera Ogawa NIH-43 and Vibrio cholera Inaba 35-A3 were prepared from rabbit hyperimmune sera by absorbing against a heterologous strain. Using ammonium sulphate precipitation procedure, gamma globulins were purified and concentrated. To visualize antigen-antibody reaction, gamma globulins were conjugated to Staphylococcus aureus cowan-l (NCTC: 8325) ...
متن کاملRapid Detection of Vibrio Cholera by a Coagglutination Complex using Staphylococcus aureus Including Protein A
Introduction: Vibrio cholera is a gram-negative bacterium that causes cholera which is an acute diarrheal illness. Cholera outbreak mostly happens in hot seasons when the bacteria reproduce rapidly in water contaminated with feces. Severe diarrhea and mortality have been the prevalent symptoms of cholera which affect the hygienic status and economic features of humans during centuries. There is...
متن کاملrapid identification of vibrio cholera-01 by coagglutination test using mono-specific antibody
mono-specific antisera against vibrio cholera ogawa nih-43 and vibrio cholera inaba 35-a3 were prepared from rabbit hyperimmune sera by absorbing against a heterologous strain. using ammonium sulphate precipitation procedure, gamma globulins were purified and concentrated. to visualize antigen-antibody reaction, gamma globulins were conjugated to staphylococcus aureus cowan-l (nctc: 8325) in th...
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We compared the Phadebact Gonococcus Test with conventional methods in identifying 210 strains of Neisseria gonorrhoeae. Of these, 209 strains were correctly identified (99.5%).
متن کاملRapid microimmunodiffusion method with species-specific antiserum raised to purified antigen for identification of Vibrio vulnificus.
An antigen common to Vibrio vulnificus strains, designated VVA, was purified by ammonium sulfate precipitation, gel filtration, ion-exchange column chromatography, and preparative gel electrophoresis. The molecular weight of VVA was 64,000 when estimated by gel filtration and 40,000 when measured by denaturing polyacrylamide gel electrophoresis. Antiserum prepared against purified VVA (anti-VVA...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1986
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.52.6.1299-1304.1986